The absence of these various germination proteins also decreased but did not abolish germination with the non-GR-dependent germinants dodecylamine and CaDPA, but spores without CwlJ exhibited no germination with CaDPA. Spores without the CLEs SleB or CwlJ exhibited greatly slowed germination with L-alanine, but spores lacking all germinant receptor proteins did not germinate with L-alanine. sporogenes spore germination with L-alanine, primarily by shortening T 1 times. sporogenes spores lacking the cortex-lytic enzyme (CLE) CwlJ spores exhibited a T lag2 in L-alanine germination. This has not been seen with spores of other species. After T release, some spores also displayed another lag in rate of change in DIC image intensity before the start of a second obvious DIC image intensity decline of 25–30% at T lag2 prior to T lys. However, variability in T 1 (or T lag1) times appeared to be the major reason for the heterogeneity between individual spores in their germination times. T 1, T lag1, T release, and T lys times for individual spores were heterogeneous, but Δ T release ( T release – T lag1) periods were relatively constant. sporogenes spores germinated with L-alanine began slow leakage of ∼5% of their large Ca-dipicolinic acid (CaDPA) depot at T 1, all transitioned to rapid CaDPA release at T lag1, completed CaDPA release at T release, and finished peptidoglycan cortex hydrolysis at T lys. sporogenes wild-type and germination mutant spores. In this study, Raman spectroscopy and differential interference contrast (DIC) microscopy were used to analyze the germination kinetics of multiple individual C. sporogenes spores requires their germination and return to life. sporogenes spores have also been proposed as a vector to deliver drugs to tumor cells for cancer treatments. botulinum spores for testing the efficacy of commercial sterilization. The Gram-positive spore-forming anaerobe Clostridium sporogenes is a significant cause of food spoilage, and it is also used as a surrogate for C.
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